Mouse Metabolism Core : Services | Rate | Schedule Use | Training | Links
Instruments within the Diabetes Center Metabolic Core include: a set of 12 metabolic chambers (Oxymax Lab Animal Monitoring System or CLAMS), a DEXA, a EchoMRI device and our new Seahorse Bioscience XFe24 Extracellular Flux Analyzer. The DEXA, EchoMRI, and Flux Analyzer are available for use, after training by any UCSF and, pending availability, non-UCSF laboratories. The CLAMS is not operated by Core users.
The UCSF Diabetes Center Metabolic Core operates four kinds of equipment:
CLAMS or Metabolic chambers: (http://www.colinst.com/brief.php?id=61)
We not analyze data for the user, we just format them (Excel file) so you have an average per hour for each metabolic parameter (VCO2, VO2, RER, food intake,...). However, I do provide extensive information and documentation on how to interpret the CLAMS data. Right now, those values are normalized to body weight (default output from the instrument). Please note: it is very important to build a cohort of animals with similar weights (below or above 30g-35g).
Food Intake Measurement System: (http://www.colinst.com/brief.php?id=61)Two independent systems of 24 metabolic chambers, to monitor Food Intake (cumulative amount of food eaten and bout of feeding) with food access control (fasting, paired feeding) (Columbus Instruments).
Experiments at thermoneutrality (30 C) (Power Scientific).
DEXA Piximus: http://piximus.com/Dual Energy X-ray Absorptiometry (DEXA) scanning is utilized to measure bone mineral density and tissue composition (fat mass & lean mass) in anesthetized mice. This device is housed in PS-644 (PS building). DEXA offers a relatively non-invasive method to measure body composition in small rodents, and it allows us to measure the body composition changes over time. The PIXImus DEXA scanner is ideal for longitudinal studies requiring repeated measurements on the same mouse. DEXA scans can be performed before and after compound administration to determine whether body composition has changed. Effects of genetic alterations, high fat feeding, surgical ovariectomy, etc. on body composition also can be measured.
EchoMRI: EchoMRI-3in1™ for Live Animals (Mice), Organs, and Biopsy:http://www.echomri.com/Body_Composition_3_in_1.aspxEcho Magnetic Resonance Imaging (EchoMRI) from Echo Medical System, Houston, TX, is used to precisely measure whole body composition parameters such as total body fat, lean mass, body fluids, and total body water in live mice without the need for anesthesia or sedation. With its high sensitivity, EchoMRI is ideal for assessing small alterations in mouse whole body composition as a result of genetic mutation or exposure to novel compounds.
Seahorse Bioscience XFe24 Extracellular Flux Analyzer:
Contact us regarding training and if you are already user, remember to sign up using the Google calendar for the Seahorse.
CENTER MEMBERS ARE REMINDED TO ACKNOWLEDGE DRC CENTER GRANT (P30 DK063720) CONTRIBUTIONS WITH ‘THIS RESEARCH WAS SUPPORTED BY GRANTS X, Y FROM NIH P30 DK063720'.