Professor
M_Microbiology and Immunology
In the Roybal Lab we harness the tools of synthetic and chemical biology to enhance the therapeutic potential of engineered immune cells. We take a comprehensive approach to cellular engineering by developing new synthetic receptors, signal transduction cascades, and cellular response programs to enhance the safety and effectiveness of adoptive cell therapies. We also study the logic of natural cellular signaling systems, and the underlying principles of cellular communication and collective cell behavior during an immune response.
Publications
Abstract A026: Deciphering natural and synthetic genetic programs governing human T cell function with combinatorial CRISPR activation and interference screens.
Cancer immunology research
CD4+ CAR T-cell exhaustion associated with early relapse of multiple myeloma after BCMA CAR T-cell therapy.
Blood advances
Designed Endocytosis-Triggering Proteins mediate Targeted Degradation.
bioRxiv : the preprint server for biology
Synthesizing a Smarter CAR T Cell: Advanced Engineering of T-cell Immunotherapies.
Cancer immunology research
Toward the clinical development of synthetic immunity to cancer.
Immunological reviews
Synthetic cytokine circuits that drive T cells into immune-excluded tumors.
Science (New York, N.Y.)
Hyperstabilization of T cell microvilli contacts by chimeric antigen receptors.
The Journal of cell biology
Multidimensional control of therapeutic human cell function with synthetic gene circuits.
Science (New York, N.Y.)
Pooled screening of CAR T cells identifies diverse immune signaling domains for next-generation immunotherapies.
Science translational medicine
Diminished cell proliferation promotes natural killer cell adaptive-like phenotype by limiting FcεRIγ expression.
The Journal of experimental medicine
Antigen-Dependent Inducible T-Cell Reporter System for PET Imaging of Breast Cancer and Glioblastoma.
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
Differential IL-12 signaling induces human natural killer cell activating receptor-mediated ligand-specific expansion.
The Journal of experimental medicine
Synthetic biology: at the crossroads of genetic engineering and human therapeutics-a Keystone Symposia report.
Annals of the New York Academy of Sciences
SynNotch CAR circuits enhance solid tumor recognition and promote persistent antitumor activity in mouse models.
Science translational medicine
SynNotch-CAR T cells overcome challenges of specificity, heterogeneity, and persistence in treating glioblastoma.
Science translational medicine
DNA scaffolds enable efficient and tunable functionalization of biomaterials for immune cell modulation.
Nature nanotechnology
Precise T cell recognition programs designed by transcriptionally linking multiple receptors.
Science (New York, N.Y.)
MM-378: Inhibitory and Stimulatory Interplay Between Myeloma Tumor and T Cells.
Clinical Lymphoma Myeloma & Leukemia
CRISPR-based screens uncover determinants of immunotherapy response in multiple myeloma.
Blood advances
convertibleCARs: A chimeric antigen receptor system for flexible control of activity and antigen targeting.
Communications biology
IMMU-21. SEQUENTIAL TWO-RECEPTOR PRIMING CAR SYSTEM TO OVERCOME HETEROGENEOUS ANTIGEN EXPRESSION.
Neuro-Oncology
Paving New Roads for CARs.
Trends in cancer
NextGen cell-based immunotherapies in cancer and other immune disorders.
Current opinion in immunology
Abstract B044: Engineering CAR NK cells for antigen-dependent autocrine expansion.
Convergence of Technology and Cancer Immunotherapy
Beyond the TCR, Antigen Discrimination in T Cells Continues in the LAT:GRB2:SOS Protein Condensate.
Biophysical Journal
IMMU-57. SEQUENTIAL TWO-RECEPTOR PRIMING CAR SYSTEM TO OVERCOME HETEROGENEOUS ANTIGEN EXPRESSION.
Neuro-Oncology
Refining cell therapy.
Science (New York, N.Y.)
Synthetic Immunology: Hacking Immune Cells to Expand Their Therapeutic Capabilities.
Annual review of immunology
CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells.
Scientific reports
Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors.
Cell
CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells.
The Journal of Immunology
Computational spatiotemporal analysis identifies WAVE2 and cofilin as joint regulators of costimulation-mediated T cell actin dynamics.
Science signaling
Cellular Structures Controlling T Cell Signaling in Time and Space.
Encyclopedia of Cell Biology
Remote control of therapeutic T cells through a small molecule-gated chimeric receptor.
Science (New York, N.Y.)
Synthetic biology approaches to engineer T cells.
Current opinion in immunology
Cellular structures controlling T cell signaling in time and space
Encyclopedia of Cell Biology
IFN-α suppresses GATA3 transcription from a distal exon and promotes H3K27 trimethylation of the CNS-1 enhancer in human Th2 cells.
Journal of immunology (Baltimore, Md. : 1950)
The actin-driven spatiotemporal organization of T-cell signaling at the system scale.
Immunological reviews
Differential regulation of the period genes in striatal regions following cocaine exposure.
PloS one
New TACTICS for finding Numb.
Immunology and cell biology
Mycobacterial shuttle vectors designed for high-level protein expression in infected macrophages.
Applied and environmental microbiology
The CD3 zeta subunit contains a phosphoinositide-binding motif that is required for the stable accumulation of TCR-CD3 complex at the immunological synapse.
Journal of immunology (Baltimore, Md. : 1950)
Tentative and transient natural killer cell polarization balances the requirements for discriminatory recognition and cytolytic efficacy.
Communicative & integrative biology
Inhibiting the inhibitor of the inhibitor: blocking PKC-theta to enhance regulatory T cell function.
Science signaling
Spatiotemporal patterning during T cell activation is highly diverse.
Science signaling
Spatiotemporal patterning of T cell signaling as an important regulator of T cell activation at the systems level.
The FASEB Journal
Mania-like behavior induced by disruption of CLOCK.
Proceedings of the National Academy of Sciences of the United States of America