Kole Roybal
Professor
M_Microbiology and Immunology

In the Roybal Lab we harness the tools of synthetic and chemical biology to enhance the therapeutic potential of engineered immune cells. We take a comprehensive approach to cellular engineering by developing new synthetic receptors, signal transduction cascades, and cellular response programs to enhance the safety and effectiveness of adoptive cell therapies. We also study the logic of natural cellular signaling systems, and the underlying principles of cellular communication and collective cell behavior during an immune response.

Publications

Ultra-large targeted DNA integrations in primary human cells.

bioRxiv : the preprint server for biology

Kernick C, Chow L, Alejandro M, Li K, Foisey MG, Yang X, Hilburger C, Lu J, Wu L, McClellan A, Takacsi-Nagy O, Brajenovic R, Theberath NE, Fuentes EC, Lin E, Hartman A, Truong T, Lee JHJ, Workley L, Ha AS, Ji Y, Putnam N, Andronikou N, Fatima N, Dotson M, Wong KA, Burns CH, Engelhard FAS, Stoyanova E, Vukovic M, Adie T, Lim WA, Roybal K, Santostefano K, Almeida R, Allen GM, Shy BR, Roth TL

In vivo site-specific engineering to reprogram T cells.

Nature

Nyberg WA, Bernard PL, Ngo W, Wang CH, Ark J, Rothrock A, Borgo GM, Kimmerly GR, Jung JH, Allain V, Hamilton JR, Baldwin A, Stickels R, Wyman S, Khan SH, Lang S, Marsh D, Almudhfar N, Novick C, Mortazavi Y, Zhang S, AbdElwakil MM, Sandoval LR, Hwang S, Chu SN, Jung H, Liu C, Sharma D, McCreary T, Li Z, Satpathy AT, Carnevale J, Rutishauser RL, Cromer MK, Roybal KT, Dodgson SE, Doudna JA, Asokan A, Eyquem J

Designed endocytosis-inducing proteins degrade targets and amplify signals.

Nature

Huang B, Abedi M, Ahn G, Coventry B, Sappington I, Tang C, Wang R, Schlichthaerle T, Zhang JZ, Wang Y, Goreshnik I, Chiu CW, Chazin-Gray A, Chan S, Gerben S, Murray A, Wang S, O'Neill J, Yi L, Yeh R, Misquith A, Wolf A, Tomasovic LM, Piraner DI, Duran Gonzalez MJ, Bennett NR, Venkatesh P, Ahlrichs M, Dobbins C, Yang W, Wang X, Sahtoe DD, Vafeados D, Mout R, Shivaei S, Cao L, Carter L, Stewart L, Spangler JB, Roybal KT, Greisen PJ, Li X, Bernardes GJL, Bertozzi CR, Baker D

Designed Endocytosis-Triggering Proteins mediate Targeted Degradation.

bioRxiv : the preprint server for biology

Huang B, Abedi M, Ahn G, Coventry B, Sappington I, Wang R, Schlichthaerle T, Zhang JZ, Wang Y, Goreshnik I, Chiu CW, Chazin-Gray A, Chan S, Gerben S, Murray A, Wang S, O'Neill J, Yeh R, Misquith A, Wolf A, Tomasovic LM, Piraner DI, Gonzalez MJD, Bennett NR, Venkatesh P, Satoe D, Ahlrichs M, Dobbins C, Yang W, Wang X, Vafeados D, Mout R, Shivaei S, Cao L, Carter L, Stewart L, Spangler JB, Bernardes GJL, Roybal KT, Greisen P, Li X, Bertozzi C, Baker D

Mania-like behavior induced by disruption of CLOCK.

Proceedings of the National Academy of Sciences of the United States of America

Roybal K, Theobold D, Graham A, DiNieri JA, Russo SJ, Krishnan V, Chakravarty S, Peevey J, Oehrlein N, Birnbaum S, Vitaterna MH, Orsulak P, Takahashi JS, Nestler EJ, Carlezon WA, McClung CA