UCSF Cell and Genome Engineering Core
The UCSF Cell and Genome Engineering Core (CGEC) combines cell culture and banking with genome engineering services; the services of the former ES Cell Targeting Core have been expanded to include CRISPR technology and are consolidated within the CGEC. Formerly provided by the Cell Culture Facility, culture and banking services are now provided by CGEC as well.
Gladstone Transgenic Gene Targeting Core
The Gladstone Transgenic Gene targeting core injects either DNA or ES cells to create genetically modified mouse models.
- Microinjection of DNA
- Microinjection of ES cells
Contact: Junli Zhang ([email protected])
UCSF Rederivation Core
The core creates new mice by embryo transfer in order to prevent the potential for transmission of pathogens.
UCSF Cryopreservation and Resuscitation Core
The core will cryopreserve sperm and embryo and, on request, resuscitate them to re-create mouse lines.
Lentiviral RNAi Core
Knowing the functions of the genes involved in a human disease helps us to better create therapies. RNA interference is a powerful way to silence a gene in a cell, allowing research investigators to study the resulting cellular phenotype. We combine the power of RNA interference and the use of lentiviral technologies to create silencing systems for research use. To view our services, click here. If you would like to use these services, simply create a new account at the ViraCore site and complete the requested fields. Creating an account gives you access to Core services including virus production, a lentivirus vector database, important biosafety information, and e-office hours for trouble-shooting your RNAi experiments.